Journal of Interdisciplinary Histopathology



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Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma

Urania Skoufi, Dimitrios L Arvanitis, Litsa Lampri, Elli Ioachim, Jim Koutsogiannis, Christina Skoufi, Dimitris Tsironis and Antigony Mitselou.

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Abstract
Objectives: Intercellular adhesion mediated by claudin and cadherin/catenin complex is a prerequisite of epithelial integrity and differentiation and has been suggested to be frequently disturbed in cancers. Endoglin (CD105) has been shown to be a more useful marker to identify proliferating endothelium involved in angiogenesis than pan-endothelial markers such as CD31. The aim of this study was to assess the relationship between these markers and clinicopathological features of colorectal carcinomas.
Materials and Methods: Surgical specimens from 69 patients with colorectal cancer were immunostained for claudin-1, E-cadherin, β-catenin, endoglin and CD31.
Results: Forty-six (66.7%), 67 (97.1%), and 67 (97.1%) of the tumors, expressed immunostaining for claudin-1, E-cadherin and β-catenin, respectively. A significant association was seen between claudin-1 and E-cadherin expression (p=0.002), as well with β-catenin (p=0.009). High β-catenin expression appeared to reduce the risk of poor outcome. Endoglin vessel expression was correlated significantly with vessel invasion (p

Key words: Colorectal cancer, claudin-1, E-cadherin, b-catenin, endoglin, CD31, immunohistochemistry


 
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REFERENCES
1. Gonzalez-Mariscal L, Betanzos A, Nava P, Jaramillo BE. Tight junction proteins. ProgBiophysMolBiol 2003; 81:1-44. [DOI via Crossref]   
2. Tsukita S, Furuse M, Itoh M. Multifunctional strands in tight junctions. Nat Rev Mol Cell Biol 2001; 2: 285-293. [DOI via Crossref]    [Pubmed]   
3. Furuse M, Sasaki H, Fujimoto K, Tsukita S. A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occluding in fibroblasts. J Cell Biol 1998; 143: 391-401. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
4. Soler AP, miller RD, Laughlin KV, Carp NZ, Klurfeld DM, Mullin JM. Increased tight permeability is associated with the development of colon cancer. Carcinogenesis 1999; 20: 1425-1431. [DOI via Crossref]    [Pubmed]   
5. Martin TA, Jiang WG. Tight junctions and their role in cancer metastasis. HistolHistopathol 2001; 16: 1183-1195. [Pubmed]   
6. Peto J. Cancer epidemiology in the last century and the next decade. Nature 2001; 411: 390-395. [DOI via Crossref]    [Pubmed]   
7. Riddell RH, Petras RE, Williams GT, Sobin LH. Tumors of the Intestines (Atlas of Tumor Pathology (AFIP) 3rd Series) American Registry of Pathology, Washington DC, pp 133-189, 2002.
8. Tokes AM, Kulka J, Paku S, Szik A, Packa C, Novak PK, Szilak K, Kiss A, Schaff A. Claudin-1, -3, and -4 proteins and mRNA expression in benign and malignant breast lesions: a research study. Breast Cancer Res 2005; 7: 296-305. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
9. Tsukuhara M, Nagai H, Karmiakito T, Kawata H, Takayashiki N, Saito K, Tanaka A. Distinct expression pattern of claudin-1 and claudin -4 in intraductal papillary mucinous tumors of the pancreas. PatholInt 2005; 55: 63-69. [DOI via Crossref]    [Pubmed]   
10. Resnick MB, Konkin T, Routhier J, Sabo E, Pricolo VE. Claudin-1 is a strong prognostic indicator in stage II colonic cancer: a tissue microarray study. Mod Pathol 2005; 18: 511-518. [DOI via Crossref]    [Pubmed]   
11. Matsuoka T, Mitomi H, Fukui N, Kanazawa H, Saito T, Hayashi T, Yao T. Cluster analysis of claudin-1 and -4, E-cadherin, and b-catenin expression in colorectal cancer. J SurgOncol 2011; 103: 674-686. [DOI via Crossref]    [Pubmed]   
12. Yoshida T, Kinugasa T, Akagi Y, Kawahara A, Romeo K, Shiratsuchi I, Riju Y, Gaotanda Y, Shirouzu K. Decreased expression of claudin-1 in rectal cancer: a factor for recurrence and poor prognosis. Anticancer Res 2011; 31: 2517-2525. [Pubmed]   
13. Shibutani M, Noda E, Maeda K, Nagahara H, Ohtani H, Hirakawa K. Low expression of claudi-1 and presence of poorly differentiated tumor clusters correlate wit poor prognosis in colorectal cancer. Anticancer Res 2013; 33: 3301-3306. [Pubmed]   
14. Iacobuzio-Donahue CA, Maitra A, Shen-Ong GI, van Heek T, Ashfaq R, Meyer R, Walter K, Berg K, Hollingsworth MA, Cameron JL, Yeo CJ, Kern SE, Goggins M, Hruban RH. Discovery of novel tumor markers of pancreatic cancer using global gene expression technology. Am J Pathol 2002; 160: 1239-1249. [DOI via Crossref]   
15. Fluge O, Bruland O, Akslen LA, Lillehaug JR, Varlang JE. Gene expression in poorly differentiated thyroid carcinomas. Thyroid 2006; 16: 161-175. [DOI via Crossref]    [Pubmed]   
16. deOliveria SS, de Oliveria IM, DeSouza V, Morgado-Diaz JA. Claudins: up-regulation in human colorectal cancer. FEBS Lett 2005; 279: 6179-61-85.
17. Gröne J, Weber B, Staub E, Heinze M, Klaman I, Pilarsky C, Hermann K, Castanos-Velez E, Röpcke S, Mann B, Rosenthal A, Buhr HJ. Differential expression of genes encoding tight junction proteins in colorectal cancer: frequent dysregulation of claudin-1, -8, and -12. Int J Colorectal Dis 2007; 22: 651-659. [DOI via Crossref]    [Pubmed]   
18. Huo Q, Kinugasa T, Wang L, Huang J, Zhao J, Shibaguchi H, Kuroki M, Tanaka T, Yamashita Y, Nabeshima K, Iwasaki H, Kuroki M. Claudin-1 protein involved in the tumorigenesis of colorectal cancer. Anticancer Res 2009; 29: 851-858. [Pubmed]   
19. Mess ST Mennigen R, Spicker T, Rijcken E, Senninger N, Hafer J, Bruewer M. Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma: upregulation of claudin -1, claudin-3, claudin-4 and β-catenin. Int J Colorectal Dis 2009; 24: 361-368. [DOI via Crossref]    [Pubmed]   
20. Bezdekova M, Brychtova S, Sedlakova E, Langova K, Brychta T, Belej K. Analysis of Snail-1, E-cadherin and claudin-1 expression in colorectal adenomas and carcinomas. Int J MolSci 2012; 13: 1632-1643. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
21. Niessen CM, Gottardi CJ. Molecular components of the adherens junctions. BiochemBiophysActa 2008; 1778: 562-571.
22. Wheelock MJ, Johnson KR. Cadherins as modulators of cellular phenotype. Annu Rev Cell Dev Biol 2003; 19: 207-235. [DOI via Crossref]    [Pubmed]   
23. vanAken E, De Wever O, Correia de Rocha AS, Marcel M. Defective E-cadherin/catenin complexes in human cancer. VirchowsAech 2001; 439: 725-731. [DOI via Crossref]   
24. Takayama T, Shiozaki H, Shibamoto S, Oka H, Kimura Y, Tamura S, Inoue M, Monden T, Ito F, Monden M. Beta-catenin expression in human cancers. Am J Pathol 1996; 148: 39-46. [Pubmed]    [PMC Free Fulltext]   
25. Hiscox S, Jiang WG. Expression of E-cadherin, alpha, beta and gamma catenin in human colorectal cancer. Anticancer Res 1997; 17: 1349-1354. [Pubmed]   
26. Ghadimi BM, Behren J, Hoffmann I, Haeusch W, Birchmeier W, Schlag PM. Immunohistological analysis of E-cadherin, alpha-, beta- and gamma-catenin expression in colorectal cancer: implication for cell adhesion and signaling. Eur J Cancer 1999; 35: 60-65. [DOI via Crossref]   
27. Takeichi M. Cadherins in cancer: implications for invasion and metastasis. CurrOpin Cell Biol 1993; 5: 806-811. [DOI via Crossref]   
28. Logan CY, Nusse R. The Wnt signaling pathway in development and disease. Ann Rev Cell Dev Biol 2004; 20: 781-810. [DOI via Crossref]    [Pubmed]   
29. Duff SE, Ki C, Garland JM, Kumar S. CD105 is important for angiogenesis: evidence and potential applications. FASEB J 2003; 17: 984-992. [DOI via Crossref]    [Pubmed]   
30. Akagi K, Ikeda Y, Sumiyoshi Y, Kimura Y, Kinoshita J, Miyazaki M, Abe T. Estimation of angiogenesis with anti-CD105 immunostaining in the process of colorectal cancer development. Surgery 2002; 131: S109-S113. [DOI via Crossref]    [Pubmed]   
31. Fonsatti E, Sigalotti L, Arslan P, Altomonte M, Maio M. Emerging role of endoglin (CD105) as a marker of angiogenesis with clinical potential in human malignancies. Curr Cancer Drug Targets 2003; 3: 427-432. [DOI via Crossref]    [Pubmed]   
32. Saad RS, Liu YL, Nathan G, Celebrezze J, Medich D, Silverman JF. Endoglin (CD105) a vascular endothelial growth factor as a prognostic marker in colorectal cancer. Mod Pathol 2004; 17: 197-203. [DOI via Crossref]    [Pubmed]   
33. Romani AN, Borghetti AF, Del Rio P, Sianesi M, Soliani P. The risk of developing metastatic disease in colorectal cancer. J SurgOncol 2006; 93: 446-455. [DOI via Crossref]    [Pubmed]   
34. Hamilton SR, Honen A. Pathology and genetics tumors of the digestive system. World Health Organization Classification of Tumors. IARC Press, Lyon, 2000.
35. Dukes CE, Bussey HJR. The spread of rectal cancer and its effect in prognosis. Br J Cancer 1958; 12: 309-320. [DOI via Crossref]   
36. Cascinu S, Georgoulias V, Kerr D, Manghan T, Labianca R, Ychou M. Colorectal cancer in the adjuvant setting: perspectives on treatment and the role of prognostic factors. AnnuOncol 2003; 14 Suppl2 :ii25-ii29.
37. Mitselou A, Ioachim E, Skoufi U, Tsironis D, Nesseris I, Lampri E, Vougiouklakis T, Briassoulis E, Arvanitis LA. Association between thrombospondin-1, angiogenesis related markers, and extracellular matrix components with colorectal cancer outcome. The Open Journal of Colorectal Cancer 2012, 5:38-46
38. Mitselou A, Skoufi U, Briasoulis E, Arvanitis D, Vougiouklakis T, Tsimofiannis KE, Ioachim E. Association of syndecan-1 with angiogenesis related markers, extracellular matrix components, and clinicopathological features in colorectal carcinoma. Anticancer Res 32: 3977-3985, 2012. [Pubmed]   
39. Mitselou A, Ioachim E, Skoufi U, Tsironis C, Tsimogiannis K E., Skoufi C, Vougiouklakis T, Briasoulis E. Predictive Role of Thymidine Phosphorylase Expression in Patients with Colorectal Cancer and its Association with Angiogenesis-related Proteins and Extracellular Matrix Components. In vivo 26: 1057-1068, 2012. [Pubmed]   
40. Dhawan P, Singh AB, Deane NG, No YR, Shiou SR, Schmidt C, Neff J, Washington MK and Beauchamp RD. Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer. J Clin Invest 2005; 115: 1765-1776. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
41. Ersoz S, Mungan S, Cobanoglu U, Turgutalp H, Ozoran Y. Prognostic importance of Claudin-1 and Claudin-4 expression in colon carcinomas. Pathol Res Prac 2011; 201: 285-289. [DOI via Crossref]    [Pubmed]   
42. Elzagheid A, Buhmeida A, Laato M, El-Faitori O, Syrjanen K, Collan Y, Pyrhonen S. Loss of E-cadherin expression predicts disease recurrence and shorter survival in colorectal carcinoma. APMIS 2012; 120: 539-548. [DOI via Crossref]    [Pubmed]   
43. Buhmeida A, Elzagheid A, Algais A. Expression of the cell-cell adhesion molecule β-catenin in colorectal carcinoma and their metastasis. APMIS 2008; 116: 1-9. [DOI via Crossref]    [Pubmed]   
44. Hörkkö TT, Klintrup K, Mäkinen JM, Näpänkangas JB, Tuominen HJ, Mäkelä J, Karttunen TJ, Mäkinen MJ. Budding invasive margin and prognosis in colorectal cancer-no direct association with beta catenin expression. Eur J Cancer 2006; 42: 964-971. [DOI via Crossref]    [Pubmed]   
45. Lugli A, Zlobec I, Minoo P, Baker K, Tornillo L, Terraciano L, Jass JR. Prognostic significance of the wnt signaling pathway molecules APC, beta-catenin and E-cadherin in colorectal cancer: a tissue microarray-based analysis. Histopathology 2007; 50: 453-464. [DOI via Crossref]    [Pubmed]   
46. Wong SCC, Lo ESF, Chan AKC, Lee KC, Hsiao WL. Nuclear beta catenin as a potential prognostic marker in patients with colorectal cancer from Hong Kong. J ClinPatholMolPathol 2003; 56: 347-352.
47. Chung GG, Provost E, Kielhorn EP, Charette LA, Smith BL, Rimm DL. Tissue microarray analysis of β-catenin in colorectal cancer shows nuclear phosphor-β-catenin is associated with a better prognosis. Clin Cancer Res 2001; 7: 4013-4020. [Pubmed]   
48. Minhajat R, Mori D, Yamasaki F, Sugita Y, Satoh T, Tokunaga O. Endoglin (CD105) expression in angiogenesis of colon cancer: analysis using tissue microarrays and comparison with other endothelial markers. Virchows Arch 2006; 448: 127-134. [DOI via Crossref]    [Pubmed]   
49. Choi HJ, Hyun MS, Jung GJ, Kim SS, Hong SH. Tumor angiogenesis as a predictor in colorectal carcinoma with special reference to mode of metastasis and recurrence. Oncology 1998; 55: 575-581. [DOI via Crossref]    [Pubmed]   
50. Bellone G, Granigui C, Vizio C, Mauri FA, Prati A, Solero D, Dughera L, Ruffini E, Gasparri G, Camandona M. Abnormal expression of endoglin and its receptor complex (TGF-β1 and TGF-β receptor II) as early angiogenic switch indicator in premalignant lesions of the colon mucosa. Int J Oncol 2010; 37: 1153-1165. [DOI via Crossref]    [Pubmed]   
51. Liu Y, Jovanovic B, Pins M, Lee C, Bergan RC. Overexpression of endoglin in human prostate cancer suppresses cell detachment, migration and invasion. Oncogene 2002; 21: 8272-8281. [DOI via Crossref]    [Pubmed]   
52. Takahashi N, Kawanishi-Tabata R, Haba A Tabata H, Haruta Y, Tsai H, Sean BK. Association of serum endoglin with metastasis in patients with colorectal, breast, and other tumors, and suppressive effect of chemotherapy on the serum endoglin. Clin Cancer Res 2001; 7: 524-532. [Pubmed]   

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1
 

How to Cite this Article
Pubmed Style

Skoufi U, Arvanitis DL, Lampri L, Ioachim E, Koutsogiannis J, Skoufi C, Mitselou DTaA. Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma. J Interdiscipl Histopathol. 2014; 2(3): 135-144. doi:10.5455/jihp.20140429121735



Web Style

Skoufi U, Arvanitis DL, Lampri L, Ioachim E, Koutsogiannis J, Skoufi C, Mitselou DTaA. Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma. www.scopemed.org/?mno=156920 [Access: June 28, 2017]. doi:10.5455/jihp.20140429121735



AMA (American Medical Association) Style

Skoufi U, Arvanitis DL, Lampri L, Ioachim E, Koutsogiannis J, Skoufi C, Mitselou DTaA. Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma. J Interdiscipl Histopathol. 2014; 2(3): 135-144. doi:10.5455/jihp.20140429121735



Vancouver/ICMJE Style

Skoufi U, Arvanitis DL, Lampri L, Ioachim E, Koutsogiannis J, Skoufi C, Mitselou DTaA. Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma. J Interdiscipl Histopathol. (2014), [cited June 28, 2017]; 2(3): 135-144. doi:10.5455/jihp.20140429121735



Harvard Style

Skoufi, U., Arvanitis, D. L., Lampri, L., Ioachim, E., Koutsogiannis, J., Skoufi, C. & Mitselou, D. T. a. A. (2014) Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma. J Interdiscipl Histopathol, 2 (3), 135-144. doi:10.5455/jihp.20140429121735



Turabian Style

Skoufi, Urania, Dimitrios L Arvanitis, Litsa Lampri, Elli Ioachim, Jim Koutsogiannis, Christina Skoufi, and Dimitris Tsironis and Antigony Mitselou. 2014. Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma. Journal of Interdisciplinary Histopathology, 2 (3), 135-144. doi:10.5455/jihp.20140429121735



Chicago Style

Skoufi, Urania, Dimitrios L Arvanitis, Litsa Lampri, Elli Ioachim, Jim Koutsogiannis, Christina Skoufi, and Dimitris Tsironis and Antigony Mitselou. "Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma." Journal of Interdisciplinary Histopathology 2 (2014), 135-144. doi:10.5455/jihp.20140429121735



MLA (The Modern Language Association) Style

Skoufi, Urania, Dimitrios L Arvanitis, Litsa Lampri, Elli Ioachim, Jim Koutsogiannis, Christina Skoufi, and Dimitris Tsironis and Antigony Mitselou. "Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma." Journal of Interdisciplinary Histopathology 2.3 (2014), 135-144. Print. doi:10.5455/jihp.20140429121735



APA (American Psychological Association) Style

Skoufi, U., Arvanitis, D. L., Lampri, L., Ioachim, E., Koutsogiannis, J., Skoufi, C. & Mitselou, D. T. a. A. (2014) Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma. Journal of Interdisciplinary Histopathology, 2 (3), 135-144. doi:10.5455/jihp.20140429121735