Journal of Interdisciplinary Histopathology



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P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases

Pankaj Baweja, Vidya Monappa, Geetha Krishnanand.

Abstract
Context: p53 tumor suppressor gene is a frequent target for mutations in a variety of tumors. The mutated gene is more stable than the normal gene and can be detected by immunohistochemical methods.
Aims: 1) To determine the expression of p53 immunohistochemically in a spectrum of benign, premalignant lesions and in various histological grades of oral squamous cell carcinoma
2) To verify if any correlation exists, between the localization and intensity of p53 staining pattern, and the degree of dysplasia.
Methods and Material: p53 expression was studied in 68 cases of oral lesions using immunohistochemistry (Dako cytomation). The location and intensity of staining was noted. Suprabasal positivity was considered as abnormal.
Results: All the benign lesions and low grade dysplasia were negative or showed only basal positivity. Suprabasal positivity increased from premalignant lesions (50%) to oral carcinomas (73.8%). In the premalignant category, intensity of p53 staining increased with increasing grades of dysplasia. In the malignant category, intensity of staining was stronger in the less differentiated tumors (68.4% vs 47.8%), while positivity was higher in better differentiated tumors (78.3% vs 68.4%). A small percentage of malignant tumors (21.4%) were negative. In 31% cases of SCC, the epithelium adjacent to the tumor which showed just hyperplasia/mild dysplasia on light microscopy, revealed suprabasal positivity.
Conclusions: Expression of p53 above the basal layer is an early event in oral carcinogenesis. Stronger staining (increasing grades of dysplasia) had greater risk of progressing into malignancy. p53 positivity is an early indicator of a developing carcinoma preceding morphological tissue alterations.

Key words: p53, oral cavity lesions, squamous cell carcinoma


 
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How to Cite this Article
Pubmed Style

Baweja P, Monappa V, Krishnanand G. P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases. J Interdiscipl Histopathol. 2013; 1(3): 113-120. doi:10.5455/jihp.20130121120725



Web Style

Baweja P, Monappa V, Krishnanand G. P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases. www.scopemed.org/?mno=31332 [Access: June 28, 2017]. doi:10.5455/jihp.20130121120725



AMA (American Medical Association) Style

Baweja P, Monappa V, Krishnanand G. P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases. J Interdiscipl Histopathol. 2013; 1(3): 113-120. doi:10.5455/jihp.20130121120725



Vancouver/ICMJE Style

Baweja P, Monappa V, Krishnanand G. P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases. J Interdiscipl Histopathol. (2013), [cited June 28, 2017]; 1(3): 113-120. doi:10.5455/jihp.20130121120725



Harvard Style

Baweja, P., Monappa, V. & Krishnanand, G. (2013) P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases. J Interdiscipl Histopathol, 1 (3), 113-120. doi:10.5455/jihp.20130121120725



Turabian Style

Baweja, Pankaj, Vidya Monappa, and Geetha Krishnanand. 2013. P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases. Journal of Interdisciplinary Histopathology, 1 (3), 113-120. doi:10.5455/jihp.20130121120725



Chicago Style

Baweja, Pankaj, Vidya Monappa, and Geetha Krishnanand. "P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases." Journal of Interdisciplinary Histopathology 1 (2013), 113-120. doi:10.5455/jihp.20130121120725



MLA (The Modern Language Association) Style

Baweja, Pankaj, Vidya Monappa, and Geetha Krishnanand. "P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases." Journal of Interdisciplinary Histopathology 1.3 (2013), 113-120. Print. doi:10.5455/jihp.20130121120725



APA (American Psychological Association) Style

Baweja, P., Monappa, V. & Krishnanand, G. (2013) P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases. Journal of Interdisciplinary Histopathology, 1 (3), 113-120. doi:10.5455/jihp.20130121120725