Journal of Interdisciplinary Histopathology



e-ISSN: 2146-8362



Journal Home | Instructions for Authors | Current Issue | Online First | Previous Issues

« Previous Article
Next Article »

Short Communication Open Access


Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano.

Cited by (1)

Abstract
Abstract
Objective: Chalkbrood is a fungal disease of honey bee brood caused by Ascosphaera apis. This disease is now found around the world and there are some indications that the incidence has increased in recent years. The diagnosis of Chalkbrood is based on the recognition of typical clinical symptoms and on the identification of the causative agent. Despite the numerous methods available for the detection of A. apis, the diagnosis of this pathology is not always easy. Methods: Between June and July 2011 an increase in mortality of honey bees was observed to some apiaries of the Caserta province (Campania region, southern Italy). Larvae (no. = 50) from brood samples without signs of disease and adult honey bees (no. = 50) from the same colonies were collected and submitted to our laboratory for routine histologic evaluation.Results and Conclusions: We demonstrate that histopathology is a sensitive and specific method for the detection and identification of Ascosphaera apis infection and has the additional advantage of being able to provide a diagnosis in larvae without signs of disease.

Key words: histopathological method, Ascosphaera apis, honey bee.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Paola Maiolino
Articles by Francesca Carella
Articles by Giovanna De Leva
Articles by Laura Rinaldi
Articles by Giuseppe Cringoli
Articles by Manuela Martano
on Google
on Google Scholar
Article Statistics
 Viewed: 2840
Downloaded: 678
Cited: 1

REFERENCES
Spiltoir CF. Life cycle of Ascosphaera apis (Pericystis apis). American Journal Botany 1955; 42: 501-508. http://dx.doi.org/10.2307/2438686

http://dx.doi.org/10.2307/2438686

Spiltoir CF, Olive LS. A re-classification of the genus Pericystis Betts. Mycologia 1955; 47: 238-244. http://dx.doi.org/10.2307/3755414

http://dx.doi.org/10.2307/3755414

Koening JP, Boush GM, Erickson EH. Isolation of the chalkbrood pathogen, Ascosphaera apis, from honeybee (Apis mellifera) surfaces, pollen loads and a water source. American Bee Journal 1987; 127: 581-583.

Hitchcock JD, Christensen M. Occurrence of Chalkbrood (Ascosphaera apis) in honeybees in the United States. Mycologia 1972; 64: 1193-1198.

http://dx.doi.org/10.2307/3758091

Kluser S, Peduzzi P. Global pollinator decline: a literature review. UNEP/GRID-Europe 2007; 5 (1): pp. 1–12.

Carrera P, Sommaragua A, Vailiti G. The development of Ascophaera apis within larvae of Apis mellifera ligustica. Journal of Apicultural Research 1987; 26: 59-63.

Skou JP. More details in support of the class Ascosphaeromycetes. Mycotaxon 1988; 31 (1): 191–198.

This Article Cited By the following articles

Histopathological findings of the midgut in European honey bee (Apis Mellifera L.) naturally infected by Nosema spp.
Vet Med Anim Sci 2014; 2(1): 4.

1
 

How to Cite this Article
Pubmed Style

Maiolino P, Carella F, Leva GD, Rinaldi L, Cringoli G, Martano M. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscipl Histopathol. 2013; 1(3): 160-162. doi:10.5455/jihp.20130128010436



Web Style

Maiolino P, Carella F, Leva GD, Rinaldi L, Cringoli G, Martano M. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). www.scopemed.org/?mno=31740 [Access: June 28, 2017]. doi:10.5455/jihp.20130128010436



AMA (American Medical Association) Style

Maiolino P, Carella F, Leva GD, Rinaldi L, Cringoli G, Martano M. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscipl Histopathol. 2013; 1(3): 160-162. doi:10.5455/jihp.20130128010436



Vancouver/ICMJE Style

Maiolino P, Carella F, Leva GD, Rinaldi L, Cringoli G, Martano M. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscipl Histopathol. (2013), [cited June 28, 2017]; 1(3): 160-162. doi:10.5455/jihp.20130128010436



Harvard Style

Maiolino, P., Carella, F., Leva, G. D., Rinaldi, L., Cringoli, G. & Martano, M. (2013) Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscipl Histopathol, 1 (3), 160-162. doi:10.5455/jihp.20130128010436



Turabian Style

Maiolino, Paola, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, and Manuela Martano. 2013. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). Journal of Interdisciplinary Histopathology, 1 (3), 160-162. doi:10.5455/jihp.20130128010436



Chicago Style

Maiolino, Paola, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, and Manuela Martano. "Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)." Journal of Interdisciplinary Histopathology 1 (2013), 160-162. doi:10.5455/jihp.20130128010436



MLA (The Modern Language Association) Style

Maiolino, Paola, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, and Manuela Martano. "Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)." Journal of Interdisciplinary Histopathology 1.3 (2013), 160-162. Print. doi:10.5455/jihp.20130128010436



APA (American Psychological Association) Style

Maiolino, P., Carella, F., Leva, G. D., Rinaldi, L., Cringoli, G. & Martano, M. (2013) Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). Journal of Interdisciplinary Histopathology, 1 (3), 160-162. doi:10.5455/jihp.20130128010436