Journal of Interdisciplinary Histopathology



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Stented Vessels: A Challenge for Histological Preparation and Microscopy

Andrea Nolte, Theresa Braun, Sven Zur Oven Krockhaus, Adelheid Munz, Ulrich Vogel, Christian Schlensak, Hans Wendel, Tobias Walker.

Abstract
Objective: The first procedure to treat blocked coronary arteries was coronary artery bypass graft surgery. In 1977, Andreas Grüntzig introduced percutaneous transluminal coronary angioplasty (PTCA). Today, several stent systems exist ranging from bare metal stents to various drug-eluting stents. Unfortunately, our understanding of the arterial reaction to stent implantation is incomplete – primarily due to technical limitations in the histological study of stented vascular tissue.
Methods: In our study, we examined different histological preparation methods based on the embedding material methacrylate. The procedure of embedding and sectioning stented porcine arteries was optimized for the specific requirements, like histochemistry, immunohistochemistry or pre-stained fluorescence. Furthermore, we used a microscopical technique described as fluorescence intensity decay shape analysis microscopy (FIDSAM) to eliminate auto-fluorescence from fluorescently labeled tissue.
Results: The sections were suitable for histochemical and immunohistochemical staining. Additionally, pre-labeled fluorescence in the porcine tissue was not lost by the embedding process. The evaluation of arterial cross sections with FIDSAM technology gave new, very important insights into the examination possibilities of fluorescently labeled tissue.
Conclusions: Future studies of the vascular response to a variety of new stent materials will provide important clues to the pathogenesis resulting in restenosis and occlusion of stents.

Key words: stent, artery, restenosis, immunohistochemistry, microscopy


 
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How to Cite this Article
Pubmed Style

Nolte A, Braun T, Krockhaus SZO, Munz A, Vogel U, Schlensak C, Wendel H, Walker T. Stented Vessels: A Challenge for Histological Preparation and Microscopy. J Interdiscipl Histopathol. 2013; 1(3): 104-112. doi:10.5455/jihp.20130306112937



Web Style

Nolte A, Braun T, Krockhaus SZO, Munz A, Vogel U, Schlensak C, Wendel H, Walker T. Stented Vessels: A Challenge for Histological Preparation and Microscopy. www.scopemed.org/?mno=32882 [Access: August 24, 2017]. doi:10.5455/jihp.20130306112937



AMA (American Medical Association) Style

Nolte A, Braun T, Krockhaus SZO, Munz A, Vogel U, Schlensak C, Wendel H, Walker T. Stented Vessels: A Challenge for Histological Preparation and Microscopy. J Interdiscipl Histopathol. 2013; 1(3): 104-112. doi:10.5455/jihp.20130306112937



Vancouver/ICMJE Style

Nolte A, Braun T, Krockhaus SZO, Munz A, Vogel U, Schlensak C, Wendel H, Walker T. Stented Vessels: A Challenge for Histological Preparation and Microscopy. J Interdiscipl Histopathol. (2013), [cited August 24, 2017]; 1(3): 104-112. doi:10.5455/jihp.20130306112937



Harvard Style

Nolte, A., Braun, T., Krockhaus, S. Z. O., Munz, A., Vogel, U., Schlensak, C., Wendel, H. & Walker, T. (2013) Stented Vessels: A Challenge for Histological Preparation and Microscopy. J Interdiscipl Histopathol, 1 (3), 104-112. doi:10.5455/jihp.20130306112937



Turabian Style

Nolte, Andrea, Theresa Braun, Sven Zur Oven Krockhaus, Adelheid Munz, Ulrich Vogel, Christian Schlensak, Hans Wendel, and Tobias Walker. 2013. Stented Vessels: A Challenge for Histological Preparation and Microscopy. Journal of Interdisciplinary Histopathology, 1 (3), 104-112. doi:10.5455/jihp.20130306112937



Chicago Style

Nolte, Andrea, Theresa Braun, Sven Zur Oven Krockhaus, Adelheid Munz, Ulrich Vogel, Christian Schlensak, Hans Wendel, and Tobias Walker. "Stented Vessels: A Challenge for Histological Preparation and Microscopy." Journal of Interdisciplinary Histopathology 1 (2013), 104-112. doi:10.5455/jihp.20130306112937



MLA (The Modern Language Association) Style

Nolte, Andrea, Theresa Braun, Sven Zur Oven Krockhaus, Adelheid Munz, Ulrich Vogel, Christian Schlensak, Hans Wendel, and Tobias Walker. "Stented Vessels: A Challenge for Histological Preparation and Microscopy." Journal of Interdisciplinary Histopathology 1.3 (2013), 104-112. Print. doi:10.5455/jihp.20130306112937



APA (American Psychological Association) Style

Nolte, A., Braun, T., Krockhaus, S. Z. O., Munz, A., Vogel, U., Schlensak, C., Wendel, H. & Walker, T. (2013) Stented Vessels: A Challenge for Histological Preparation and Microscopy. Journal of Interdisciplinary Histopathology, 1 (3), 104-112. doi:10.5455/jihp.20130306112937